Cleaning bioburden with ultrasonics?
ear Sally: Please, I need help!
Can ultrasonic waves affect the sample placed in the plastic bag filled with the water? Plastic bag is in the glass beaker but there is no fluid in it. Can ultrasonic waves affect sample if it is just wetted but there is no water in the plast bag and the bag is in the glass beaker without water? I am working with bioburden and using ultrasonic to pick microorganisms from my samples.
Thank for helping me
Gordana C
Dear Gordana,
Bad news. You will have to fill the bag for there to be any appreciable retransmission of ultrasonic energy. As long as we can go from water to a hard surface, preferably stainless steel, then back to water, you can expect to see activity in the innermost container. You have a couple of things working against you here. First of all, your innermost container is a plastic bag, and second, it is not flooded. To get this done, you will need to come up with a way to process your parts with the bag filled with water.